![]() ![]() We distinguish the algorithms in terms of image quality, performance, usability and computational requirements. We evaluate these methods over large 3D microscopy images using simulated datasets and real experimental images. We examine several standard algorithms used in deconvolution microscopy, notably: Regularized inverse filter, Tikhonov regularization, Landweber, Tikhonov–Miller, Richardson–Lucy, and fast iterative shrinkage-thresholding. In this paper, we take advantage of the release of DeconvolutionLab2 to provide a complete description of the software package and its built-in deconvolution algorithms. Freely distributed, DeconvolutionLab hosts standard algorithms for 3D microscopy deconvolution and drives them through a user-oriented interface. To make deconvolution more accessible, we have developed a practical platform for deconvolution microscopy called DeconvolutionLab. Among others, end users have to select the appropriate algorithm, calibration and parametrization, while potentially facing demanding computational tasks. Unfortunately, dealing with deconvolution tools is not straightforward. ![]() Deconvolution is widely used to restore fine details of 3D biological samples. The purpose of deconvolution microscopy is to compensate numerically for this degradation. Endoscopy 45:79–85.Images in fluorescence microscopy are inherently blurred due to the limit of diffraction of light. Li Z, Zuo XL, Li CQ, Zhou CJ, Liu J, Goetz M, Kiesslich R, Wu KC, Fan DM, Li YQ (2013) In vivo molecular imaging of gastric cancer by targeting MG7 antigen with confocal laser endomicroscopy. Shahid MW, Crook JE, Meining A, Perchant A, Buchner A, Gomez V, Wallace MB (2011) Exploring the optimal fluorescein dose in probe-based confocal laser endomicroscopy for colonic imaging. Wallace MB, Fockens P (2009) Probe-based confocal laser endomicroscopy. Goetz M, Watson A, Kiesslich R (2011) Confocal laser endomicroscopy in gastrointestinal diseases. Sakashita M, Inoue H, Kashida H, Tanaka J, Cho JY, Satodate H, Hidaka E, Yoshida T, Fukami N, Tamegai Y, Shiokawa A, Kudo S (2003) Virtual histology of colorectal lesions using laser-scanning confocal microscopy. CLE can increase the diagnostic accuracy in a number of gastrointestinal pathologies.Īdvanced endoscopic imaging Cellvizio Confocal laser endomicroscopy In vivo microscopy Optical histology pCLE. It also aids in the differentiation and classification of colorectal polyps, indeterminate biliary strictures, and pancreatic cystic lesions.ĬLE has an excellent safety profile. It has been shown to increase the detection of dysplastic Barrett's esophagus, gastric intraepithelial neoplasia/early gastric cancer, and dysplasia associated with inflammatory bowel disease when compared to standard screening protocols. The technology offers an excellent safety profile with rare adverse events related to the use of fluorescent agents. FDA) Manufacturer And User facility and Device Experience (MAUDE) database was searched for reports regarding the device malfunction or injuries. ![]() The United States Food and Drug Administration (U.S. The manufacturer's website was also used to identify key references. Case reports and small case series were excluded. ![]() Bibliographies of key references were searched for relevant studies not covered by the PubMed search. The literature search used combinations of the keywords: confocal laser endomicroscopy, pCLE, Cellvizio, in vivo microscopy, optical histology, advanced endoscopic imaging, and optical diagnosis. The Society of American Gastrointestinal and Endoscopic Surgeons (SAGES) Technology and Value Assessment Committee (TAVAC) performed a PubMed/Medline database search of clinical studies involving CLE in May of 2018. There has been numerous studies evaluating this technology in gastrointestinal endoscopy, our aim was to evaluate the safety, value, and efficacy of this technology in the gastrointestinal tract. CLE technology has been used in different organ systems including the gastrointestinal tract. By using intravenous or topical fluorescent agents, CLE highlights certain mucosal elements that facilitate an optical biopsy in real time. Confocal laser endomicroscopy (CLE) is a novel endoscopic adjunct that allows real-time in vivo histological examination of mucosal surfaces. ![]()
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